Immunology Laboratory


KEY FACULTY

Barbara Detrick

Barbara Detrick, Ph.D.
Professor of Pathology
Director, Cytokine Laboratory
Primary Appointment in Pathology; Joint Appointment in Medicine and in Molecular Microbiology & Immunology (BSPH); Member, Graduate Program in Immunology; Member, Graduate Program in Molecular Microbiology and Immunology (BSPH)

600 N. Wolfe Street, Meyer B-121D
Baltimore, MD 21287
Phone: 410-614-3286/301-480-2988
E-mail:


Clinical interests
My professional interests focus on the discovery and implementation of diagnostic laboratory tests to evaluate patients with a variety of immune based disorders.

Research interests
My research interests include work on the immunologic and virologic aspects of immune mediated disorders. Currently, I investigate immune and viral factors associated with pathogenic processes within the eye. I have studied the retinal pigment epithelial (RPE) cell extensively as an important immunoregulatory cell and tracked its role in ocular immunity. Over the years, both animal model systems and a human RPE cell model have been utilized to study viral immunopathology. We have developed a murine coronavirus model, experimental coronavirus retinopathy, which identifies how a virus can trigger a retinal degenerative process. These studies revealed both a genetic predisposition and an immune component in this disease. We are continuing to explore the genetic and molecular basis of this autoimmune component of retinal tissue damage.

RPE cells are key players in the first-line defense against invading organisms. Another area of active research is the exploration of innate immunity within the retina. TLRs are critical recognition receptors in the host defense against microbial pathogens and play pivotal role in innate immunity. Using real time PCR analysis of TLR gene expression, we identified the presence of TLRs (TLRs 1-7 and 9-10) in human RPE cells. Moreover, TLR signaling in the RPE cells results in the production of several cytokines, chemokines and adhesion molecules. We are continuing our studies to further define the role of this epithelial cell in innate and adaptive immune responses.

More recently, research efforts have been focused on role of cytokines in regulating and modulating immune responses and immune mediated diseases. Specifically, I have been studying these molecules in vasculitis, infection, cardiovascular disease & atherosclerosis and transplant rejection. To date, our studies are attempting to bring together the current information concerning the role of cytokines in the development, progression, and complications of these inflammatory conditions and to identify their possible role as biomarkers of early inflammatory disease.


Recent Publications

Book
Detrick B, Schmitz J, Hamilton RG. Manual of Molecular and Clinical Laboratory Immunology. 8th Ed. ASM Press, Washington, DC. May 2016.



Selected Articles

  1. Hooks J, Nagineni C, Hooper L, Hayashi K, and Detrick B. IFN-beta Provides Immuno-protection in the Retina by Inhibiting ICAM-1 and CXCL9 in Retinal Pigment Epithelial Cells. J. Immunol. 2008, 180 (6) 3789-96.
  2. Detrick B and Hooks JJ. Immune Regulation in the Retina. Immunologic Research, 2010. 47: 153-161.
  3. Morrell, C.N., Srivastava, K., Swaim, A., Lee, M.T., Chen, J., Nagineni, C., Hooks, J.J., Detrick, B. Beta interferon suppresses the development of experimental cerebral malaria. Infect Immuno. 2011. 79 (4): 1750-8.
  4. Karafin MS, Humphrey RL and Detrick B. Evaluation of monoclonal and oligoclonal gammopathies in a pediatric population in a major urban center. Am J Clin Pathol. 2014. 141: 482-7.
  5. Chin MS, Hooper LC, Hooks JJ and Detrick B. Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR). J. Neuroimmunol. 2014; 272:42-50.
  6. Nagineni CN, Kommineni VK, Ganjbaksh N, Nagineni KK, Hooks JJ, Detrick B. Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration. Aging Dis. 2015 Nov 17;6(6):444-55.
  7. Hooks JJ, Chan CC, Sen HN, Nussenblatt RN and Detrick B. Monitoring Autoimmune Reactivity in the Retina. In Manual of Molecular and Clinical Laboratory Immunology. Eds. Detrick B, Schmitz J, Hamilton RG. ASM Press, Washington, DC. P 998 - 103, 2016.
  8. Gupta A, Montepiedra G, Gupte A, Zeldow B, Jubulis J, Detrick B, Violari A, Madhi S, Bobat R, Cotton M, Mitchell C, Spector S; IMPAACT NWCS113 and P1041 Study Team. Low Vitamin-D Levels Combined with PKP3-SIGIRR-TMEM16J Host Variants Is Associated with Tuberculosis and Death in HIV-Infected and -Exposed Infants. PLoS One. 2016 Feb 12;11(2):e0148649. doi: 10.1371/journal.pone.0148649.
  9. McGrath-Morrow SA, Collaco JM, Detrick B, Lederman HM. Serum Interleukin-6 Levels and Pulmonary Function in Ataxia-Telangiectasia. J Pediatr. 2016 Apr;171:256-261
  10. Wang GC, Han C, Detrick B, Casolaro V, Levine DM, Fried LP, Walston JD. Herpesvirus Infections and Risk of Frailty and Mortality in Older Women: Women's Health and Aging Studies. J Am Geriatr Soc. 2016 64(5):998-1005.


Richard L. Humphrey, M.D.
Associate Professor School of Medicine, Department of Pathology

Johns Hopkins Medicine
600 N. Wolfe Street, Meyer B125F
Baltimore, MD 21287;
Phone: 410-955-4382;
Fax:410-614-7314;
E-mail:

Education and Training
M.D., University of Michigan, Ann Arbor; Residency and Internship, Internal Medicine, Johns Hopkins Hospital; Fellowship, Biochemistry and Biophysics, Johns Hopkins University School of Medicine

Clinical Interests
Nationally known in the field of plasma cell dyscrasias. His work with these disorders has applications for the recognition of multiple myeloma as well as Waldastrom’s macroglobulinemia, primary amyloidois and other lymphoproliferative disorders.

Research Interests
Monoclonal gammopathies, plasma cell dyscrasias, immunoglobulin structure and function, bone marrow transplantation, amyloidosis, hyperviscosity diagnosis and clinical syndromes, cryoglobulinemia, protein electrophoresis and immunofixation.

Notable Accomplishments
Introducedcellulose acetate electrophoresis, immunoglobulin quantitation, immunoelectrophoresis to Johns Hopkins Medical Institutions. Participated in the early studies leading to myeloablative therapy and bone marrow transplantation for multiple myeloma.

Major Professional Leadership
Founding member of the Johns Hopkins Immunology Council. Served on several national task forces for cancer education and nursing research. Medical consultant for the American Red Cross, Plasma Operations. Reviewer for Medicine, Johns Hopkins Medical Journal, and Transplant Proceedings, among other professional journals.



Robert G. Hamilton, Ph.D.
Professor of Medicine and Pathology
Departments of Medicine (Division of Clinical Immunology) and Pathology
Director, Johns Hopkins Dermatology, Allergy and Clinical Immunology Reference Laboratory

Johns Hopkins Medicine,
Johns Hopkins Asthma and Allergy Center
5501 Hopkins Bayview Circle
Baltimore, MD 21224
Phone: 410-550-2031
Fax: 410-550-2030
E-mail:

Education and Training
Ph.D., Johns Hopkins University; Diplomate, American Board of Science in Nuclear Medicine; American Society of Nuclear Medicine; American Board of Medical Laboratory Immunology; American Society for Microbiology.

Clinical Interests
Allergic and Infectious disease; Immunodeficiency

Research Interests
Humoral immune responses to allergens, immunogens (vaccines) and
Assessment of Environments for Allergen burdens
Evaluation of Patients for Drug Allergy; Latex Allergy

Notable Accomplishments
Development of serologic assays for quantitation of allergen-specific human IgE, IgG, IgG1, IgG2, IgG3, and lgG4 antibodies. Co-licensee for natural rubber latex diagnostic puncture skin test reagent. Developer, novel heterologous system employing chimeric antibodies for calibration of human antibody standards. Recognized internationally as expert in humoral immune responses associated with pathology and protection of humans with allergic disease.

Major Professional Leadership
Interagency Committee on Validation of Alternative. Toxicological Methods, NIOSH, CDC. Co-Chair, Diagnostic Allergy Sub-committee, Diagnostic Immunology Committee, National Committee on Clinical Laboratory Standards. Director, Diagnostic Allergy Interlaboratory Quality Control Program for USA. Member, Clinical and Diagnostic Immunology Resource Committee, College of American Pathologists. Editorial board member of numerous professional journals, including Journal of Allergy and Clinical Immunology. Annals of Allergy, Asthma and Immunology. Journal of Clinical Immunoassay. Journal of Immunoassay. Clinical and Diagnostic Immunology. Reviewer, Journal of Clinical Biochemistry, Clinical Chemistry, Immunology Letters, Journal of Immunology, Allergy, International Archives of Allergy and Immunology.

Recent publications



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