Anatomic Pathology Consult Page | Head and Neck Pathology

Human Papillomavirus

The human papillomavirus (HPV) is a causative agent for a subset of carcinomas arising from the mucosal lining of the head and neck. A better understanding of the role HPV as a cancer causing agent tumors may provide new ways to effectively prevent and treat HPV-related cancers of the head and neck. Clearly, accurate detection of HPV in the cancer samples will be necessary to determine which patients will most likely benefit from HPV-directed therapy.

The Head and Neck Pathology Consultation Service at the The Johns Hopkins Hospital now provides a very reliable test to detect the presence HPV-16, the most common HPV subtype to cause cancers of the head and neck. The powerful HPV-16 in situ hybridization catalyzed signal amplification method (DAKO) is highly sensitive, is applicable to formalin-fixed paraffin-embedded clinical samples, and permits direct visualization of viral sequences within tissues.

HPV-16 detection is a service that is now provided to referring physicians. To send a sample for HPV-16 analysis, please follow these simple guidelines:

Johns Hopkins Medical Laboratories
1620 McElderry Street
Reed Hall Room 315
Baltimore, MD 21205
Phone 410-955-2405
Fax 410-614-7712

IMPORTANT NOTE - Unstained Slides Requirement
Note that all tissue (unstained slides) must be submitted on a plus charged slide. The Immunopathology Laboratory recommends a white plus charged slide that is available from Cardinal Health. The catalogue number for the white plus slide is M6159-6, however one must specify to the vendor whether to order as one gross (pack of 144 slides), one case (10 packs per case).