Blood Disorders (Special Coagulation Laboratory)


The Coagulation Laboratory is one of the nation's foremost resources for special coagulation studies. State-of-the-art tests performed here enhance diagnostic capabilities for congenital and acquired bleeding disorders, hyper coagulable evaluations, platelet disorders, and autoimmune coagulation disorders.

We advise physicians and others seeking help with diagnoses and patient care and provide services to other reference laboratories and support clinical trials. We have extensive experience advising on and processing data for large clinical trials, including some with thousands of patients. We offer specialized testing for supporting trials in anticoagulation.


Experience

The Division of Special Coagulation continues a distinguished research tradition. Pioneering discoveries in coagulation made at the Johns Hopkins Medical Institutions include the discovery of heparin, the elucidation of the role of platelets in hemostasis, and the lupus anticoagulant. Coagulation research continues today both in Hopkins research laboratories and clinics. This permits new research discoveries to be applied to studying causes of thrombosis or bleeding in individual patients or in supporting large multicenter clinical trials.


Expertise

Expert Consultation
Director of the Coagulation Laboratory, Dr. Thomas S. Kickler is interested in platelet disorders, hemostasis, and thrombosis. Providing medical consultation and interpretation of test results is Dr. Michael Streiff, Professor of Medicine. Dr Gang Zheng is an authority on molecular genetics and electron microscopy of platelets.

Special capabilities and services
Our fully automated lab, with four comprehensive analyzers, allows us to handle more than 500 samples a day. We are the only reference lab in this region, and one of the few nationally, able to do quantitative platelet analysis with whole blood aggregation including both molecular DNA-based tests and platelet aggregation studies. Our Department of Pathology Electron Microscopy laboratory provides the facilities to perform electron microscopy for inherited platelet disorders. Our testing facilities also include automated testing assays, molecular diagnosis capabilities, PCR equipment, and flow cytometry. coagulation testing. We maintain internal relational databases on all accounts, and can store and manage large amounts of data for clinical trials. We have special interest in antiphospholipid antibody testing and drug induced thrombocytopenia, including heparin antibody testing.


Timely Results

Most test results can be supplied in one or two days, or within hours if needed. Results can be provided by phone, fax, or e-mail. Our physicians are available for 24/7 discussion of results. A hard copy of the finished report is sent to requesting physicians and entered into our central laboratory computer system.


Faculty

Dr. Thomas Kickler

Thomas S. Kickler, M.D.

Phone:410-955-6315
Fax:410-502-1493
E-mail:

Education and Training

Professional Interests
Evaluation and development of new test procedures to evaluate hemostasis disorders using molecular, immunologic and biochemical approaches. Development of new technology to reduce the expense of coagulation testing. Evaluation of the sensitivity and specificity of hemostatic parameters in different diagnostic groups. Development of critical pathways to diagnose and treat bleeding and thrombotic disorders. Study of the role of coagulation proteins and platelets in acute and chronic transplantation rejection.

» Dr. Thomas Kickler's Papers
» Hemostasis Manual

 


Tests

To order, call Customer Service at 1-800-997-5475 between 8:00 a.m. and 11:00 p.m.

Abnormal PT and/or APTT Evaluation
This panel is used to evaluate the cause of an abnormal protime or activated partial thromboplastin time.

APTT
APTT Mixing Studies
Hepzyme (Test for heparin contamination)
PT (Prothrombin Time)
PT (Prothrombin Time) Mixing Studies
Platelet Neutralization


Cardiovascular Panel
Test abnormalities in this panel correlate with an increased risk of cardiovascular disorders.

Factor VII Activity
Fibrinogen Activity
Homocysteine (special collection)
Lipoprotein (a)
Plasminogen Activator Inhibitor-1 (PAI-1)
Prothrombin Fragment 1.2
Tissue Plasminogen Activator Antigen


Circulating Anticoagulant and Lupus Anticoagulant
A basic screening panel for factor deficiencies and the detection of inhibitors.

APTT
Mixing Studies (APTT)
Mixing Studies (PT)
PT (Prothrombin Time)
Platelet Neutralization
d-RVVT, with confirm
Anti-cardiolipen Antibody, IgG, IgM, IgA
Anti-B2 GP1 antibodies, IgG, IgM, IgA


DDAVP Challenge
The panel is used to evaluate the response of mild hemophilia and von Willebrand disease patients to DDAVP.

Factor VIII Activity
Ristocetin CoFactor assay
WF Antigen
HEME-8 (pre - post)


DIC Screen
The DIC screen is useful in the diagnosis and follow-up of disseminated intravascular coagulation.

APTT
D-dimer (Quantitative)
Fibrinogen
Prothrombin Time


Extrinsic Pathway Panel
Panel used to evaluate and identify the cause of an abnormal prothrombin time.

Factor VII Activity
Factor X Activity
Factor V Activity
Factor II Activity


Fibrinolysis Panel
The fibrinolysis panel may be used to monitor or diagnose acquired or congenital abnormalities of the fibrinolytic system.

alpha-2-Antiplasmin
D-dimer Quantitative
Fibrinogen Degradation Products
Plasminogen Activator Inhibitor-1 (PAI-1)
Plasminogen Activity
Tissue Plasminogen Activator Antigen


Intrinsic Pathway Panel
Panel can be used to evaluate and identify the cause of an abnormal activated partial thromboplastin time.

Factor XII Activity
Factor XI Activity
Factor IX Activity
Factor VIII Activity


Lupus Anticoagulant (Antiphospholipid Screen)
A basic screening panel for the detection of lupus-like inhibitors. The profile may also be used for patients with an unexplained prolonged prothrombin time or APTT.

Anticardiolipin Antibody (ELISA)
APTT and Mixing Studies
D-RVVT (Dilute Russell Viper Venom) and Mixing Studies
PT (Prothrombin Time) and Mixing Studies
Platelet Neutralization Test if evidence for an inhibitor


Platelet Function Panel
Panel used to identify platelet function problems.

PFA-100
Bleeding Time
Clot Retraction
Platelet Aggregation
Platelet Secretion (ATP release)
Flow cytometry studies if indicated
Prothrombin Consumption Test


Thrombotic Risk Panel - Comprehensive
The thrombotic risk panel is a comprehensive group of tests for evaluating patients with hypercoagulable states.

Activated Protein C Resistance and Factor V Leiden
Antithrombin III Activity
Dilute Russell's Viper Venom (D-RVVT)
Fibrinogen Activity, Fibrinogen Antigen
Homocysteine (special collection)
Lipoprotein (a)
Lupus Anticoagulant Screen
Plasminogen Activator Inhibitor 1 (PAI-1)
Plasminogen Activity
C Activity
Protein C Antigen
Protein S Activity Protein S Antigen Total
Thrombin Time
Tissue Plasminogen Activator Antigen
Prothrombin Gene Mutation


Thrombotic Risk Screen
A group of basic tests used to screen patients for only the most common causes of thrombosis.

Activated Protein C Resistance
Antithrombin III Activity
D-RVVT
Homocysteine (special collection)
Protein C Activity
Protein S Activity


von Willebrand Panel
This panel is for the evaluation of suspected von Willebrand disease.

APTT
Factor VIII Activity
Ristocetin Cofactor
vWF Antigen
vWF Multimers as needed


Markers of Hyper-Coagulation

Prothrombin fragment 1,2
Plasma p-Selectin
Thromboxane
Beta-Thromboglobulin
PF4


Miscellaneous Bleeding Disorders

Factor XIII Activity




Special Ordering Instructions

Collection and Processing
Tests for hemostatic evaluations are extremely sensitive to variations in sample collection and specimen processing. Test results are directly affected by the quality of the sample presented for analysis. Therefore, it is extremely important that NCCLS reference guidelines for coagulation sample collection and processing are followed.

Specimens may be collected in siliconized evacuated tubes or by the two syringe technique. The specimen must not be the first tube drawn, a traumatic venipuncture or hemolyzed. When samples are collected from patients with indwelling catheters, the first 20 mls of blood should not be used for coagulation testing. If the catheter contains heparin, the first 30 mls should not be used for coagulation testing.

Citrated Plasma

Serum for Homocysteine Testing
Note: Improper sample collection may invalidate the result.


Serum for Heparin Induced Antibody


Platelet Factor 4 and Beta Thromboglobulin
Note: Use Diatube--break the vacuum of these tubes before sample collection.


Shipping

Note: Plasma and serum specimens must be frozen prior to shipping. Purple top tubes should be sent in separate box with cold packs.