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The Genetics of Pancreatic Cancer

-- Technical Section

 

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ALK5 Primers

EXON 1

5’-END 5’-CGTCCTCCGAGCAGTTAC-3

3’-END 5’-CGCCGCTCACCCGTCG-3’

seq primer 5’-ctccgagcagttacaaagg-3’

 

EXON 2

3’END 5’-CTAGAAAAAAATTTATACAACTTAC-3’

5’END 5’-attttttctagaatctttctctttt-3’

seq primer:5’-CAAACTGTTAACCTTGAGATTTT-3’

 

EXON 3

5’ END 5’-GGGGGTTGCCACCTACAG-3’

3’ END 5’-AAAAAAGGAGAAACAATTATG-3’

seq primer 5’-ggtgttcatgtttatttcactc-3’

 

EXON 4

5’ END 5’-AAATTCCTTAAAGGGATAGC-3’

3’ END 5’-TATAACATAAGGAAAAGCAAATG-3’

seq primer 5’-atgtaatattgttgattgtgttgag-3’

 

EXON 5

5’END 5’-TAAATAAACAAGTTACCAATAATG-3’

3’ END 5’-TGTTTAAAGCTTAAATAATAGAAC-3’

seq primer 5’-TTAAAGCTTAAATAATAGAACtgctt-3’

 

EXON 6

5’ END 5’-GTGATTGGTATTACCTTTTAAG-3’

3’ END 5’-AAAAAAGGAGATTTCAGAAGATA-3’

seq primer 5’-agaagatattaaatatagttgttc-3’

 

EXON 7

5’ END 5’-TGTACATATGTCTATGTATAAG-3

3’ END 5’-TCTGGCACTCGGTGACATC-3’

seq primer 5’-TCTGGCACTCGGTGACATC-3’

 

EXON 8

5’ END 5’CTCTCAGGTGATCTTTTAATG-3’

3’ END 5’-tccttaaagaaaagtagcaaac-3’

seq primer. 5’-AAGCAGTTTAGTTTAGAAAAATTG-3’

 

EXON 9

5’ END 5’-TTTTTTTTTTATATTTTCTTGTAG-3’

3’ END 5’-GCAAAAGGAAGCAATATCCTTC-3’

seq primer: 5’-agagttcaggcaaagctgtag-3’