The Sol Goldman Pancreatic Cancer Research Center

The Genetics of Pancreatic Cancer

Representational Difference Analysis (RDA) Protocol

GENERATION OF AMPLICONS

  1. Digestion genomic DNA
  2. Ligation to RBamHI primers
  3. PCR-1 amplicons
  4. Size selection tester amplicons
  5. PCR-2 amplicons
  6. Digestion amplicons
  7. Ligation tester amplicons to JBamHI primers

SUBTRACTIVE DNA HYBRIDIZATION

  1. Hybridization-1
  2. PCR-1 of Hyb-1
  3. Mung bean nuclease treatment Hyb-1
  4. PCR-2 of Hyb-1

Abbreviations, solutions and procedures