H. Benjamin Larman, Ph.D.
Primary Appointment in Pathology
The Larman laboratory develops and deploys new technologies to study the human immune system. Much of our work utilizes 'next generation' DNA sequencing to enable novel, massively parallel molecular assays. Examples of these technologies include:
- phage display of synthetic peptidome libraries for comprehensive, quantitative profiling of antibodies;
- ribosome display of ORFeome libraries for antigen discovery, protein-protein interaction studies, and drug target identification;
- ultrasensitive, multiplex RNA quantification techniques to monitor gene expression and detect microbes;
- pooled genetic screening to elucidate immune cell function and identify new therapeutic targets.
The laboratory uses these and other unbiased approaches to identify new opportunities for monitoring and manipulating immune responses.
Larman HB, Zhao Z, Laserson U, Li MZ, Ciccia A, Gakidis MAM, Church GM, Kesari S, LeProust EM, Solimini NL & Elledge SJ, “Autoantigen discovery with a synthetic human peptidome,” Nat. Biotech. 2011; 29(6): 535-41
Larman HB*, Salajegheh M*, Nazareno R, Lam T, Sauld J, Steen H, Kong SW, Pinkus JL, Amato AA, Elledge SJ & Greenberg SA, "Cytosolic 5′-nucleotidase 1A autoimmunity in sporadic inclusion body myositis," Ann. Neurol. 2012; 73(3): 408-18
Zhu J*, Larman HB*, Gao G, Somwar R, Zheng W, Laserson U, Ciccia A, Pavlova N, Church GM, Kesari S & Elledge SJ, "Protein interaction discovery using parallel analysis of translated ORFs (PLATO)," Nat. Biotech. 2013; 31(4): 331-4
Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A & Schultz PG, "Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay," Nucleic Acids Res. 2014; 42(14): 9146-57.
Xu GJ, Kula T, Xu Q, Li MZ, Vernon SD, Ndung’ T, Ruxrungtham K, Sanchez J, Brander C, Chung R, O’Connor KC, Walker B, Larman HB, & Elledge SJ, “Comprehensive serological profiling of human populations using a synthetic human virome,” Science. 2015; 384(6239): aaa0698-1-9