Mark A. Marzinke, Ph.D.
Primary Appointment in Pathology; Secondary Appointment in Medicine; Associate Director, Clinical Pharmacology Analytical Laboratory (Division of Clinical Pharmacology)
My primary research focuses are in the development and validation of analytical methods for the assessment of phenotype-genotype and pharmacokinetic-pharmacodynamic (PK-PD) relationships. Specifically, my interests lie in the clinical application of molecular testing for targeted pharmacogenetic analysis using a variety of techniques, including high-resolution melting (HRM) curve discrimination of genetic polymorphisms, and probe-based quantitative PCR techniques to identify genetic aberrations, to direct drug treatment. Additionally, my work is focused on the quantification or qualitative identification of small molecules, including anti-retroviral agents and pain management medications in a variety of specimen sources to assess PK-PD relationships. Our group utilizes mass spectrometry in a variety of ways, including the development, bioanalytical validation and utilization of liquid chromatographic-tandem mass spectrometric (LC-MS/MS) and high-resolution accurate mass (HRAM) spectrometric methods for analyte identification and quantification. Other research interests include laboratory automation and workflow and process improvement initiatives.
Marzinke MA, Choi CH, Chen L, Shih IM, Chan DW, Zhang H. Proteomic Analysis of Temporally Stimulated Ovarian Cancer Cells for Biomarker Discovery. Mol Cell Proteomics 2013; 12(2): 356-368.
Marzinke MA, Clarke W, Wang L, Cummings V, Liu TY, Piwowar-Manning E, Breaud A, Griffith S, Buchbinder S, Shoptaw S, Del Rio C, Magnus M, Mannheimer S, Fields SD, Mayer KH, Wheeler DP, Koblin BA, Eshleman SH, Fogel JM. Nondisclosure of HIV Status in a Clinical Trial Setting: Antiretroviral Drug Screening Can Help Distinguish Between Newly Diagnosed and Previously Diagnosed HIV Infection. Clin Infect Dis. 2014 Jan;58(1):117-20. PMID: 24092804.
Seserko LA, Emory JF, Hendrix CW, Marzinke MA. The development and validation of an UHPLC-MS/MS method for the rapid quantification of the antiretroviral agent dapivirine in human plasma. Bioanalysis. 2013; 5(22):2771-83. PMID: 24256358.
Petrides AK, Clarke W, Marzinke MA. Application and utility of pharmacogenetics in the clinical laboratory. J Analyt Molecul Tech. 2014; 1(1): 15.
Marzinke MA, Breaud A, Parsons TL, Cohen MS, Piwowar-Manning E, Eshleman SH, Clarke W. The development and validation of a method using high-resolution mass spectrometry (HRMS) for the qualitative detection of antiretroviral agents in human blood. Clin Chim Acta, 2014; 433C: 157-168.