Ordering Information

Epic Orderable Name: CD19/CD20 B Lymphocytes

Epic Test Id: LAB20437

Testing Includes Percent of CD20+ Lymphocytes (for Anti-CD20 therapy patients only) with CD19 B Lymphocytes

Testing Schedule Monday thru Saturday: 8AM

Turnaround Time 2 Days

Order Priorities Routine   Stat

Performing Laboratory JHH Flow Cytometry Laboratory (WGB 2300) 410-614-2968

LOINC 11130-2

Methodology Immunophenotyping

Ordering Restrictions STOP; DON'T COLLECT sample between Sat 7AM and Sun 7AM (Mon 7AM if MON is a holiday). Samples will be cancelled because sample will be too old at time of testing, compromising sample integrity. NEVER refrigerate. Transport at room temperature only. If cold or on ice, specimen will be cancelled. Specimen must be received within 30.0 hours.

Down-time Requisition JHH Pathology 10 Flow Requisition

Specimen Requirements

Specimen STOP; DON'T COLLECT sample between Sat 7AM and Sun 7AM (Mon 7AM if MON is a holiday). Samples will be cancelled because sample will be too old at time of testing, compromising sample integrity. NEVER refrigerate. Transport at room temperature only. If cold or on ice, specimen will be cancelled. Specimen must be received within 30.0 hours from collection.

Collection Container Lavender Top Tube

Collection Volume 2.0 mL

Minimum Volume 3.0 mL

Specimen Stability Testing needs to be completed within 30.0 hours of collection. Never refrigerate. Store at room temperature only.

Send Specimen To JHH Flow Cytometry Laboratory (WGB 2300)/410-614-2968

Specimen Types Blood

Reference Ranges & Interpretation

Reference Range

0-3 mo. 6-32%

3-6 mo. 11-41%

6-12 mo. 14-37%

1-2 yr. 16-35%

2-6 yr. 14-33%

6-12 yr. 13-27%

12-18 yr. 6-23%

>18 yrs. 6-19%

For Lab Staff

Performing Laboratory JHH Flow Cytometry Laboratory (WGB 2300) 410-614-2968

Performing Workstation JLAB FLOW CYTOMETRY

Processing Aliquot Volume 1.0 mL

Minimum Aliquot Volume 1.0 mL

Processing (in Performing Lab) Transport Room Temperature Whole Blood to Flow Cytometry Lab Tube Station 141.

Comments Test includes Percent of CD20+ Lymphocytes (for Anti-CD20 therapy patients only) with CD19 B Lymphocytes

PHLEBOTOMY STATUS: Routine Rounds

LABORATORY NOTES: Please indicate if patient is on Anti-CD20 therapy. Specimen will be canceled if refrigerated or not processed within

24 hours of collection. Call Flow Cytometry lab @ 410-614-2968 for additional instructions.

Minimum Aliquot Volume is 1.0 mL.

JHH SPECIAL HANDLING: Transport Whole Blood at Room Temperature to T Cell Subsets laboratory (Weinberg 2306) or if using the pneumatic tube system,

Tube Station 141,within 24 hours of collection.

PROCESSING INSTRUCTIONS FOR OUTREACH SITES: Deliver Whole Blood at Room Temperature to Flow Cytometry lab (Weinberg 2306).