Ordering Information

Epic Orderable Name: TCell Subsets

Epic Test Id: LAB20432

Testing Includes Percent of CD3+ Lymphocytes

Percent of CD8+CD3+ Lymphocytes

Percent of CD4+CD3+ Lymphocytes

Absolute # CD4+CD3+ Lymphocytes

Ratio of CD4+/CD8+

Order Priorities Routine

Performing Laboratory JHH Flow Cytometry Laboratory (WGB 2300) 410-614-2968

LOINC 65759-3 

Ordering Restrictions STOP; DON'T COLLECT sample between Sat 7AM and Sun 7AM (Mon 7AM if MON is a holiday). Samples will be cancelled because sample will be too old at time of testing, compromising sample integrity.

Specimen Requirements

Collection Container Lavender Top Tube

Collection Volume 3.0 mL

Minimum Volume 3.0 mL

Specimen Stability Testing needs to be completed within 30.0 hours of collection. Never refrigerate. Store at room temperature only.

Specimen Types Blood

Reference Ranges & Interpretation

Reference Range

CD8% Reference Ranges

0-3 mo. 12-28%

3-6 mo. 12-23%

6-12 mo. 12-24%

1-2 yrs. 14-30%

2-6 yrs. 16-30%

6-12 yrs. 18-35%

12-18 yrs. 18-35%

18-23 yrs. 10-39%

> 23 yrs. 10-36%

CD4% Reference Ranges

0-3 mo. 35-64%

3-6 mo. 35-56%

6-12 mo. 31-56%

1-2 yrs. 32-51%

2-6 yrs. 28-47%

6-12 yrs. 31-47%

12-18 yrs. 31-52%

18-23 yrs. 28-57%

> 23 yrs. 32-68%

CD3% Reference Ranges

0-3 mo. 53-84%

3-6 mo. 51-77%

6-12 mo. 49-76%

1-2 yrs. 53-75%

2-6 yrs. 56-75%

6-12 yrs. 60-76%

12-18 yrs. 56-84%

18-23 yrs. 55-83%

> 23 yrs. 51-91%

Absolute CD4 Reference Ranges / cu mm

0-3 mo. 1600-4000

3-6 mo. 1800-4000

6-12 mo. 1400-4300

1-2 yrs. 1300-3400

2-6 yrs. 700-2200

6-12 yrs. 650-1500

12-18 yrs. 530-1300

18-23 yrs. 300-1400

> 23 yrs. 458-1344

For Lab Staff

Performing Laboratory JHH Flow Cytometry Laboratory (WGB 2300) 410-614-2968

Performing Workstation JLAB FLOW CYTOMETRY

Processing Aliquot Volume 1.0 mL

Minimum Aliquot Volume 1.0 mL

Processing (in Performing Lab) Deliver Room Temperature Whole Blood to Flow Cytometry Lab (Weinberg 2306).

Comments Test includes: Percent of CD3+ Lymphocytes, Percent of CD8+CD3+Lymphocytes,Percent of CD4+CD3+ Lymphocytes, Absolute # CD4+CD3+ Lymphocytes, Ratio of CD4+/CD8+

PHLEBOTOMY STATUS: Routine Rounds

JHH SPECIAL HANDLING: Transport Whole Blood at Room Temperature to T Cell Subsets laboratory (Weinberg 2306)or if using the pneumatic tube system,

Tube Station 141,within 24 hours of collection. Call Flow Cytometry lab @ 410-614-2968 for additional instructions.

PROCESSING INSTRUCTIONS FOR OUTREACH SITES: Deliver Whole Blood at Room Temperature to Flow Cytometry lab (Weinberg 2306).

REFERENCE RANGE:

CD4% : AGE Percent ABSOLUTE CD4 : AGE /cumm

0- 3 mo. 35-64% 0- 3 mo.

1600-4000

3- 6 mo. 35-56% 3- 6 mo.

1800-4000

6-12 mo. 31-56% 6-12 mo.

1400-4300

1- 2 yrs. 32-51% 1- 2 yrs.

1300-3400

2- 6 yrs. 28-47% 2- 6 yrs.

700-2200

6-12 yrs 31-47% 6-12 yrs.

650-1500

12-18 yrs. 31-52% 12-18 yrs.

530-1300

8-23 yrs. 28-57% 18-23 yrs.

300-1400

>23 yrs. 32-68% >23 yrs.

458-1344

CD3% : AGE Percent CD8% : AGE

PERCENT

0- 3 mo. 53-48% 0- 3 mo.

12-28%

3- 6 mo. 51-77% 3- 6 mo.

12-23%

6-12 mo. 49-76% 6-12 mo.

12-24%

1- 2 yrs. 53-75% 1- 2 yrs.

14-30%

2- 6 yrs. 56-75% 2- 6 yrs.

16-30%

6-12 yrs. 60-76% 6-12 yrs.

18-35%

12-18 yrs. 56-84% 12-18 yrs.

18-35%

18-23 yrs. 55-83% 18-23 yrs.

10-39%

>23 yrs. 51-91% >23 yrs.

10-36%