Ordering Information

Epic Orderable Name: CD4+CD3+ Lymphocytes

Epic Test Id: LAB20433

Testing Includes Percent of CD3+ Lymphocytes

Percent of CD4+CD3+ Lymphocytes

Absolute # CD4+CD3+ Lymphocytes

Testing Schedule Monday thru Saturday: 8AM

Turnaround Time 2 Days

Order Priorities Routine  Stat

LOINC 24467-3

Ordering Restrictions

STOP; DON'T COLLECT sample between Sat 7AM and Sun 7AM (Mon 7AM if MON is a holiday). Samples will be cancelled because sample will be too old at time of testing, compromising sample integrity. Must be received within 30.0 hours of collection. Do not refrigerate. Transport at room temperature only.

If ordering this test on the eve before a Holiday OR between Sat 7AM and Sunday 7AM (Mon 7AM if Monday is a holiday), change the order’s START DATE to the NEXT BUSINESS DAY. Samples collected during these times will be CANCELLED because the sample will be too old at the time of testing, compromising sample integrity.

Down-time Requisition JHH Pathology 10 Flow Requisition

Specimen Requirements

Collection Container Lavender Top Tube

Collection Volume 3.0 mL

Minimum Volume 1-2 mL

Specimen Stability Must be received within 30.0 hours of collection. Do not refrigerate. Transport at room temperature only.

Send Specimen To JHH Flow Cytometry Laboratory (WGB 2300)/410-614-2968

Specimen Types Blood

Reference Ranges & Interpretation

Reference Range

CD4% Reference Ranges

0-3 mo. 35-64%

3-6 mo. 35-56%

6-12 mo. 31-56%

1-2 yrs. 32-51%

2-6 yrs. 28-47%

6-12 yrs. 31-47%

12-18 yrs. 31-52%

18-23 yrs. 28-57%

>23 yrs. 32-68%

CD3% Reference Ranges

0-3 mo. 53-84%

3-6 mo. 51-77%

6-12 mo. 49-76%

1-2 yrs. 53-75%

2-6 yrs. 56-75%

6-12 yrs. 60-76%

12-18 yrs. 56-84%

18-23 yrs. 55-83%

> 23 yrs. 51-91%

Absolute CD4 Reference Ranges / cu mm

0-3 mo. 1600-4000

3-6 mo. 1800-4000

6-12 mo. 1400-4300

1-2 yrs. 1300-3400

2-6 yrs. 700-2200

6-12 yrs. 650-1500

12-18 yrs. 530-1300

18-23 yrs. 300-1400

> 23 yrs. 458-1344

For Lab Staff

Performing Workstation JLAB FLOW CYTOMETRY

Processing Aliquot Volume 1.0 mL

Minimum Aliquot Volume 1.0 mL

Processing (in Performing Lab) Deliver Room Temperature Whole Blood to Flow Cytometry Lab (Weinberg 2306). Requisition is NOT needed to add on T-Cells from Customer Service.

Comments

PHLEBOTOMY STATUS: Routine Rounds

LABORATORY NOTES: Specimen will be canceled if refrigerated or not processed within 24 hours of collection. Call Flow Cytometry lab @ 410-614-2968 for additional instructions.

Minimum Aliquot Volume is 1.0 mL.

JHH SPECIAL HANDLING: Transport Whole Blood at Room Temperature to T Cell Subsets laboratory (Weinberg 2306)or if using the pneumatic tube system,

Tube Station 141,within 24 hours of collection.

PROCESSING INSTRUCTIONS FOR OUTREACH SITES: Deliver Whole Blood at Room Temperature to Flow Cytology lab (Weinberg 2306).

REFERENCE RANGE:

CD4%: AGE Percent ABSOLUTE CD4; AGE /cumm

0- 3 mo. 35-64% 0- 3 mo. 1600-4000

3- 6 mo. 35-56% 3- 6 mo. 1800-4000

6-12 mo. 31-56% 6-12 mo. 1400-4300

1- 2 yrs. 32-51% 1- 2 yrs. 1300-3400

2- 6 yrs. 28-47% 2- 6 yrs. 700-2200

6-12 yrs 31-47% 6-12 yrs. 650-1500

12-18 yrs. 31-52% 2-18 yrs. 530-1300

18-23 yrs. 28-57% 18-23 yrs. 300-1400

>23 yrs. 32-68% >23 yrs. 458-1344

CD3% : AGE Percent

0- 3 mo. 53-48%

3- 6 mo. 51-77%

6-12 mo. 49-76%

1- 2 yrs. 53-75%

2- 6 yrs. 56-75%

6-12 yrs. 60-76%

12-18 yrs. 56-84%

18-23 yrs. 55-83%

>23 yrs. 51-91%