Ordering Instructions
To order, call consult office at 410-955-2405 between 8:00 a.m. and 5:00 p.m.
Our Services
We offer interpretations of standard and specialized studies by experienced specialists of both native kidney and allograft pathology.
Standard Renal Biopsy Processing
The laboratory provides expert standard processing of native kidney and allograft renal biopsies, using light microscopy with immunofluorescence and/or electron microscopy as appropriate for each case. Studies can be done on tissue or on blocks of slides prepared elsewhere.
Available Tests
Light Microscopy (LM) - H&E, trichrome, PAS, and silver stains
Immunofluorescence (IF) - Immunoglobulins, complement components, light chains, Alport panel (alpha chains of type 4 collagen), IgG subclasses
Electron microscopy (EM)
Turnaround Time
| Order Type | Turnaround Time |
|---|---|
| Routine processing and interpretation for light microscopy and immunofluorescence | 1 day |
| Routine processing and interpretation for electron microscopy | 5 days |
| Rush processing for LM and IF, on request | 4 to 6 hours |
| Electron microscopy | 2 days if needed |
Specialized Studies
Studies are available as needed on routinely processed specimens or can be applied to slides or tissue blocks sent in consultation.
Tests
Immunoperoxidase (immunoglobins, immunoglobulin light chains, immunoglobulin heavy chains, amyloid AA, viral antigens, myoglobin, cell surface markers) Immunoelectron microscopy
Preparation of EM specimens from paraffin-embedded material or tissue sections on slides
In situ hybridization (viral antigens)
Turnaround Time
| Order Type | Turnaround Time |
|---|---|
| Immunoperoxidase and in situ hybridization | 1 to 2 days |
| Immunoelectron microscopy | 3 to 4 days |
Mailing & Delivery
Mailing Address
Forward slides by courier or express mail to:
Johns Hopkins Medical Laboratories
1620 McElderry Street
Reed Hall Rm 315
Baltimore, MD 21205
Phone 410-955-2405
Fax 410-367-2404
To send biopsies, e-mail us at: [email protected]
Delivery Information
Deliveries Monday through Friday 8am-4:30pm
Deliver to:
Johns Hopkins Medical Institutions
Department of Pathology
ATTN: Surgical Pathology - Renal
Pathology Bldg 700
600 N. Wolfe Street
Baltimore, MD 21287
For questions or concerns during delivery please call the following in order:
- 410-502-7260 Surgical Pathology Accessioning (Renal)
- 410-955-2386 Renal Pathology Administration
- 410-283-3316 Renal Attending On Call (pager)
- * When using the pager number please enter a complete telephone number where to return the call.
After-Hours/Weekends
Deliveries after hours and weekends
Johns Hopkins Medical Institutions
Department of Pathology
ATTN: Surgical Pathology - Renal
401 N. Broadway
2nd Floor Security Desk
Baltimore, MD 21231-2410
For questions or concerns during delivery please call the following in order:
410-283-3316 (Renal Attending - On-Call Pager)
410-283-9563 (Renal Tech - On-Call Pager)
* When using the pager number please enter a complete telephone number where to return the call.
For weekend biopsies an advanced call and/or follow up call is recommended.
Preparing the Biopsy for Transport
- Place part of the biopsy in 10% buffered formalin, another part in Michel's transfer medium (also called Zeus medium),and another glutaraldehyde each in a small, tightly closed specimen container. We order our medium from Wampole Laboratories, Division of Carter-Wallace, Inc., Cranbury, NJ 08512.
- For shipping, please use ice, NOT dry ice.
- Frozen specimens will have a large amount of artifact and may not be interpretable.
- Please include a form with clinical information for each; we provide a standardized form for this purpose.